Longevity & Cellular Renewal
NAD+
Nicotinamide adenine dinucleotide
β-Nicotinamide Adenine Dinucleotide
Research context
NAD⁺ functions as an electron carrier in redox reactions and as a consumed substrate for sirtuin deacylases, poly(ADP-ribose) polymerases, and CD38. Research applications include enzyme kinetics requiring defined cofactor concentration, and studies of NAD⁺ pool depletion and restoration in cultured systems.
Common assays: Enzymatic cycling assay · LC-MS/MS quantification · Sirtuin activity coupling
Specification
| Designation | Nicotinamide adenine dinucleotide |
|---|---|
| Full name | β-Nicotinamide Adenine Dinucleotide |
| CAS number | 53-84-9 |
| Molecular formula | C21H27N7O14P2 |
| Molecular weight | 663.43 g/mol |
| Formats | 10 mL Vial · 15 mL Advanced Liquid Peptide Spray |
| Solubility | Water, aqueous buffer |
| Storage | Lyophilised: −20 °C, desiccated, protected from light |
| Regulatory status | Not an approved drug. Listed in FDA 503A Category 1 (under evaluation) as of October 2026. 2026 status by compound → |
| Current lot | NAD500N0526 → |
NAD+ COA: certificates of analysis by lot
4 lots of NAD+ have a published certificate of analysis from AxisPharm, LLC, an independent laboratory. Each certificate reports the laboratory's results for that lot.
| Lot | Format | Purity (HPLC-UV) | Reported |
|---|---|---|---|
| NAD500N0526 | 500 mg Spray | 99.5% | 2026-05-27 |
| NAD500VCS0126 | 500 mg Vial | >99.5% | 2026-02-20 |
| NAD500VAS0126 | 500 mg Vial | >99.5% | 2026-02-20 |
| NAD500N1125 | 500 mg Spray | >99.0% | 2025-11-13 |
Published literature
Selected peer-reviewed references describing research involving this compound. Listed for reference only; inclusion is not a claim about any outcome.
- Cantó C, Menzies KJ, Auwerx J.NAD+ metabolism and the control of energy homeostasis: a balancing act between mitochondria and the nucleusCell Metabolism · 2015 Find on PubMed ↗
- Verdin E.NAD+ in aging, metabolism, and neurodegenerationScience · 2015 Find on PubMed ↗
Common questions
Why does NAD⁺ purity matter particularly in enzyme work?
NAD⁺ degrades to nicotinamide and ADP-ribose, both biologically active — nicotinamide inhibits sirtuins. Degradation products confound results, which is why free nicotinamide is reported separately on the certificate.
How stable is lyophilised NAD⁺?
It is hygroscopic and light-sensitive. Storage at −20 °C under desiccation is standard, and lots are handled and shipped accordingly.
Where can I see the NAD+ certificate of analysis?
Every published NAD+ lot has its own certificate page on this site, listed under "NAD+ COA" above. There are 4 published lots; the most recent, lot NAD500N0526, was reported on 2026-05-27 at 99.5% purity by HPLC-UV. The certificates are issued by AxisPharm, LLC, an independent laboratory.
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